In the Porter-Silber test for hydroxycorticosteroids, which reagent is used and what color is observed?

In the Porter-Silber test for hydroxycorticosteroids, which reagent is used and what color is observed?

• A. Pink
• B. Yellow
• C. Purple
• D. Blue

Answer: (B)

Porter-Silber is a colorimetric test for hydroxycorticosteroids in urine. When these steroids are present, they react with the Porter-Silber reagent to give a yellow coloration. The yellow color indicates a positive result for hydroxycorticosteroids in the sample, whereas other colors would not be expected for this test. This test is used as a qualitative screen in adrenal steroid assessment.

Porter-Silber is a colorimetric test for hydroxycorticosteroids in urine. When these steroids are present, they react with the Porter-Silber reagent to give a yellow coloration. The yellow color indicates a positive result for hydroxycorticosteroids in the sample, whereas other colors would not be expected for this test. This test is used as a qualitative screen in adrenal steroid assessment.